Ex Vivo Coagulation Test on Tissue Factor-Expressing Cells with a Calibrated Automated Thrombogram

URI http://harp.lib.hiroshima-u.ac.jp/hirokoku-u/metadata/4719
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Title
Ex Vivo Coagulation Test on Tissue Factor-Expressing Cells with a Calibrated Automated Thrombogram
Author
氏名 高宮 脩
ヨミ タカミヤ オサム
別名 Takamiya Osamu
氏名 阪田 光彦
ヨミ サカタ ミツヒコ
別名 Sakata Mitsuhiko
Subject
Ex vivo test
Thrombin generation
Tissue factor-bearing cell
Coagulation factor-deficient plasma
Calibrated automated thrombography
Abstract

A calibrated automated thrombogram is not affected by the turbidity of platelet and cell preparations because the measurement is based on fluorescence. To examine conditions that mimic the physiological state, we investigated thrombograms that show thrombin generation on tissue factor (TF)-bearing cells. An increase in the number of J82 cells did not affect the endogenous thrombin potential (ETP) of normal plasma, although the lag time (LT), the peak height, and the time to peak (ttPeak) did depend on cell concentration. When 5 parameters of coagulation factor-deficient plasmas were plotted on a radar graph, the thrombogram pattern of factor XI (FXI)-deficient plasma became slightly reduced. The thrombogram did not improve when washed normal platelets or washed normal platelets with adenosine diphosphate (ADP) were added. FVII-depleted plasma, FVIII-deficient plasma, and FIX-deficient plasma showed remarkably reduced peak heights, ttPeaks, and times to the end of thrombin generation (start tails). The thrombogram of FVII-depleted plasma was characterized by a remarkably prolonged LT, unlike the patterns of FVIII- or FIX-deficient plasma and FXI-depleted plasma. The ETP of FVIII- and FIX-deficient plasma, but not FVII-depleted plasma, improved significantly upon addition of washed normal platelets or washed normal platelets with ADP. The thrombograms of coagulation factor-deficient plasma containing TF-bearing cells differed from those for recombinant TF and phospholipid in the liquid phase. We suggest that thrombograms using TF-bearing cells can be a useful ex vivo test, because this experimental model may be analogous to most coagulation processes in vivo.

Description

論文に記載されている著者名は O.TAKAMIYA, M.SAKATA

Journal Title
Laboratory hematology : official publication of the International Society for Laboratory Hematology.
Volume
14
Issue
4
Spage
39
Epage
44
Published Date
2008
Publisher
Kluge Carden Jennings Pub. Co.
ISSN
1080-2924
NCID
AA11859936
Language
eng
NIIType
Journal Article
Text Version
出版社版
Old URI
Set
hirokoku-u