ヘモグロビンに吸収される標識赤血球の蛍光波長が赤血球速度計測に及ぼす影響

URI http://harp.lib.hiroshima-u.ac.jp/hirokoku-u/metadata/6825
File
Title
ヘモグロビンに吸収される標識赤血球の蛍光波長が赤血球速度計測に及ぼす影響
Author
氏名 山本 明美
ヨミ ヤマモト アケミ
別名 Yamamoto Akemi
氏名 中野 厚史
ヨミ ナカノ アツシ
別名 Nakano Atsushi
氏名 南山 求
ヨミ ミナミヤマ モトム
別名 Minamiyama Motomu
Subject
hemoglobin absorption of fluorescent markers
blood flow measurement
microvessel
intra-vital video microscope
Abstract

Flow velocity of red blood cell (Vrbc) in microvessels provides the important, information of circulatory functions. Because fluorescence intensity of fluorescent marker of red blood cell (RBC) decrease by hemogobin in the microvessel, it is expected that the fluorescent marker at the central position in blood flow is hard to be detected. In present study, we analyzed the absorbance difference in fluorescent markers on the measurement of Vrbc. Anesthetized rats were used during spontaneous breathing. The mesentery was extended in the organbath on a stage of an intra-vital fluorescence video microscope. Topical application of fluorescent dye (di-8-ANEPPS)was carried out at the mesentery. On the other hand, load of the fluorescent probe was carried out to RBC,and then marker RBC returned into the vein. We could get simultaneously two wavelengths images on a same fluorescence screen by using a W-view optical system,and we obtained an image at 560 nm (green image) and that at 620 nm (red image) on a same video screen. We compared the Vrbc profiles between on the green image and the red image in a venule. The Vrbc in green image was slower than that in red image at same position in the venule. We cloud predict that it was better to measure Vrbc on the red image than the green image.

Journal Title
医療工学雑誌
Issue
4
Spage
17
Epage
23
Published Date
2010/03/15
Publisher
広島国際大学大学院総合人間科学研究科医療工学専攻
ISSN
1881-9265
NCID
AA12217173
Language
jpn
NIIType
Departmental Bulletin Paper
Text Version
出版社版
Sort Key
3
Old URI
Set
hirokoku-u