hemoglobin absorption of fluorescent markers
blood flow measurement
intra-vital video microscope
Flow velocity of red blood cell (Vrbc) in microvessels provides the important, information of circulatory functions. Because fluorescence intensity of fluorescent marker of red blood cell (RBC) decrease by hemogobin in the microvessel, it is expected that the fluorescent marker at the central position in blood flow is hard to be detected. In present study, we analyzed the absorbance difference in fluorescent markers on the measurement of Vrbc. Anesthetized rats were used during spontaneous breathing. The mesentery was extended in the organbath on a stage of an intra-vital fluorescence video microscope. Topical application of fluorescent dye (di-8-ANEPPS)was carried out at the mesentery. On the other hand, load of the fluorescent probe was carried out to RBC，and then marker RBC returned into the vein. We could get simultaneously two wavelengths images on a same fluorescence screen by using a W-view optical system，and we obtained an image at 560 nm (green image) and that at 620 nm (red image) on a same video screen. We compared the Vrbc profiles between on the green image and the red image in a venule. The Vrbc in green image was slower than that in red image at same position in the venule. We cloud predict that it was better to measure Vrbc on the red image than the green image.
Departmental Bulletin Paper