Laminaria japonica Areschougの皮層細胞における微小部X線分析

URI http://harp.lib.hiroshima-u.ac.jp/hju/metadata/5092
File
Title
Laminaria japonica Areschougの皮層細胞における微小部X線分析
Title Alternative
X-ray Microanalysis of the Cortical Cells in Laminaria japonica Areschoug
Author
氏名 奥田 弘枝
ヨミ オクダ ヒロエ
別名 OKUDA Hiroe
Subject
Laminaria japonica Areschoug
皮層細胞
NDC
498.5
Abstract

Energy-dispersive X-ray microanalysis (EDX) of a tangle (Laminaria japonica Areschoug) was performed using an analytical electron microscope (JEM 1200EX-Tracor Northern TN2000).
1. Fresh tangle was dried in the sun and fractured by a razor blade. A small piece of fractured tissue was placed on a holder, coated with carbon in vacuum, and examined at scanning electron microscopic (SEM) range. Some dried samples were soaked in distilled water for 60 minutes. The soaked and not-soaked samples were fixed with glutaraldehyde and osmium tetroxide before being dehydrated and embedded in Epon. Semithin sections were obtained, and examined at scanning-transmission electron microscope (STEM) range. Results are as follows:
Fractured specimens of dried tangle were adequate for EDX-SEM analysis to show exact contents of all contained elements.
2. X-ray peaks of C1, K, Na, Mg, P and S were detected in the cortical cells and their cell walls of untreated samples.
3. X-ray peaks of C1 K, Na, Mg, P and S were disappeared in the soaked tangle.
4. Ca element localized in the cell walls, but was not extracted, probably because of its tight bound to some polysaccharides in the cell wall structure.

Journal Title
広島女学院大学論集
Volume
38
Spage
211
Epage
225
Published Date
1988-12
Publisher
広島女学院大学
ISSN
03748057
Language
jpn
NIIType
Departmental Bulletin Paper
Text Version
出版社版
Old URI
Set
hju