The Effect of Several Fixing Solutions on the Fixation Image and Staining of the Lilac Leaf (Syringa Vulgaris)

URI http://harp.lib.hiroshima-u.ac.jp/hju/metadata/5627
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Title
The Effect of Several Fixing Solutions on the Fixation Image and Staining of the Lilac Leaf (Syringa Vulgaris)
Author
氏名 HIRAHARA Sachiye
ヨミ ヒラハラ サチエ
別名 平原 幸枝
Subject
Staining
Lilac Leaf
NDC
596
Abstract

The purpose of this study was to determine the effect of several killing agents on samples cut from the lilac leaves (Syringa
vzugaris). The leaves were cut in four different lengths to indicate the different treatments to be given to them. The killing solutions used were CRAF III,FAA II,Allen-Bouin II and a control with no kil1ing
agent. A procedure for embedding in paraffin was used to provide one sample from each of the four treatments on each microscopic slide. The slides were stained with Safranine-O and Fast-Green; Safranine-O and Anilin Blue; Safranine-O and Bismarck Brown and Fast-Green; Safranine-O and Delafield's Hematoxylin; Conant's Triarch Quadruple and Heidenhain's Iron-Alum Hematoxyiin. The sections were examined to
note the effect of the four treatments on the fixation image and on the staining. From the standpoints of both the fixation image and staining,the findings indicate preference for treatment in the following order CRAF III,Allen-Bouin II,FAA II and no ki1ling agent.

Journal Title
広島女学院大学論集
Volume
12
Spage
169
Epage
176
Published Date
1962-12
Publisher
広島女学院大学
ISSN
0374-8057
Language
eng
NIIType
Departmental Bulletin Paper
Text Version
出版社版
Old URI
Set
hju