Inhibitory effect of cadmium on competitive nodulation ability of Bradyrhizobium japonicum

URI http://harp.lib.hiroshima-u.ac.jp/it-hiroshima/metadata/12083
File
Title
Inhibitory effect of cadmium on competitive nodulation ability of Bradyrhizobium japonicum
Author
氏名 Ozawa Takashi
ヨミ オザワ タカシ
別名 小澤 隆司
氏名 Ijiri Kenji
ヨミ イジリ ケンジ
別名 Ijiri Kenji
Subject
cadmium
competitiveness
hydrophobicity
legume
lipopolysaccharide
rhizobium
Abstract

Competitive nodulation abilities of Bradyrhizobium japonicum strains USDA110ET and A1017ET were significantly depressed by growing the strains in yeast extract-mannitol broth supplemented with 2μM CdCl2. Soybean seedlings were co-inoculated with each test strain and its competitor strain 138NR, and the bacteria in nodules formed on 21 days old plant roots were identified by the antibiotic resistant markers. Cell surface hydrophobicity of each test strain increased by growing the strains with 2μM CdCl2, though definite increase in bacterial attachment to plant root surface was hindered by excessive secretion of exopolysaccharides of the strains. Polyacrylamide gel electrophoresis revealed that growing the test strains in the medium with 2μM CdCl2 induced the production of lipopolysaccharides of small molecular sizes. The results in this study suggest that cadmium of low concentration causes the weakening of competitive nodulation ability of rhizobia through inhibition of the lipopolysaccharide synthesis.

Journal Title
広島工業大学紀要. 研究編
Volume
49
Spage
27
Epage
33
Published Date
2015-02
ISSN
1346-9975
NCID
AA11599110
Language
jpn
NIIType
Departmental Bulletin Paper
Text Version
出版社版
Set
it-hiroshima