Polyamine analyses in capsicum plants by a combined method of thin-layer chromatography and image analysis
thin layer chromatography
Polyamines are widely distributed in living organisms and essential for their cell proliferation and cell differentiation. Although it has shown in recent decdes that polyamines have specific funtions in plants, such as environmental stress response and organ development, the molecular basis still remains unclear. A previous study has reported that polyamines are highly abundant in green peppers. In this study, to examine whether polyamines are abundant in the every cultivars of capsicum plants, we analyzed the amount of polyamines, which are putrescine, spermidine, and spermine, by a combined method of thin-layer chromatography and image analysis. Taking an organ-specific of polyamine accumulation into consideration, our results may suggest that high accumulation of putrescine in green peppers is caused by a competitive inhibition due to ethylene biosynthesis.