ニホンウナギ卵形成過程での総 SODと亜鉛の変化

URI http://harp.lib.hiroshima-u.ac.jp/it-hiroshima/metadata/12385
ファイル
タイトル
ニホンウナギ卵形成過程での総 SODと亜鉛の変化
別タイトル
Mechanisms underlying stress tolerance during oogenesis in Japanese eel.
著者
氏名 楠目 峻大
ヨミ クスメ タカヒロ
別名 Takahiro KUSUME
氏名 新 大樹
ヨミ アタラシ ダイキ
別名 Daiki ATARASHI
氏名 三浦 智恵美
ヨミ ミウラ チエミ
別名 Chiemi MIURA
キーワード
stress, superoxide dismutase, oogenesis, zinc, organ culture system
抄録

The presence of the antioxidant enzyme Cu/Zn-superoxide dismutase (SOD)and zinc in spermatogonia of Japanese eel has been shown to play an important role in the antioxidant system of germ cells. The purpose of this study was to clarify the mechanisms involved in the stress tolerance during oogenesis. The total SOD activity and the zinc levels, measured by a competitive inhibition assay with a tetrazolium salt, and zinc fluorescent probe, respectively, were found to decrease as the oocyte developed into more advanced stage. By employing an organ culture system, zinc deficiency induced by an intracellular zinc chelating agent (TPEN) was observed to cause atresia of follicle cells. Addition of zinc ions attenuates TPEN-induced atresia. These results imply a relationship between SOD and zinc, and suggests an involvement of zinc in stress tolerance of follicle cells.

掲載雑誌名
広島工業大学紀要. 研究編
55
開始ページ
19
終了ページ
23
出版年月日
21-Feb
出版者
広島工業大学
ISSN
1346-9975
NCID
AA11599110
本文言語
日本語
資料タイプ
紀要論文
著者版フラグ
出版社版
区分
it-hiroshima