緩速ろ過池に生息する微小動物の腸内細菌フローラの系統解析

URI http://harp.lib.hiroshima-u.ac.jp/kure-nct/metadata/12006
File
Title
緩速ろ過池に生息する微小動物の腸内細菌フローラの系統解析
Title Alternative
Molecular Analysis on Gut Flora of Aquatic Invertebrates Inhabiting Slow Sand Filter Beds
Author
氏名 竹内 準一
ヨミ タケウチ ジュンイチ
別名 Takeuchi Juni
氏名 黒杭 祐太
ヨミ クロクイ ユウタ
別名 Kurokui Yuta
氏名 田中 陵暉
ヨミ タナカ リョウキ
別名 Tanaka Ryoki
Subject
normal flora
Aeolosoma
Tubifex
Chironomus
Aeromonas
PCR
正常菌相
アブラミミズ
イトミミズ
ユスリカ
エアロモナス
遺伝子増幅
Abstract

Internal colonizers of bacteria were isolated from gut after partial surface sterilization using UV irradiation and the subsequent dispersal treatment. Genomic DNA extraction from each isolate was performed with a FTA TM card (Whatman, UK), and then PCR-amplified, targeting 16S rDNA fragments with universal primer pairs. The PCR products were purified and then sequenced to identify a series of bacterial strains collected during this study. Most of the gut floras were members of the Aeromonas group commonly distributed in aquatic habitats, whilst others were identified as Paucibacter and Acidovorax, both may degrade native and manmade toxic materials such as cyanotoxin and dioxin, respectively. These normal floras of invertebrates inhabiting slow sand filters contribute not only to purify water but may decompose unfavorable toxic substances, presumably reducing potential health risks during drinking water production.

Journal Title
呉工業高等専門学校研究報告
Issue
75
Spage
13
Epage
18
Published Date
2013
Publisher
呉工業高等専門学校
ISSN
0286-4037
Language
jpn
NIIType
Departmental Bulletin Paper
Text Version
出版社版
Set
kure-nct