チューブリン相互作用によるTetrahymena繊毛外腕ダイニン頭部の構造変化

URI http://harp.lib.hiroshima-u.ac.jp/pu-hiroshima/metadata/12357
File
Title
チューブリン相互作用によるTetrahymena繊毛外腕ダイニン頭部の構造変化
Title Alternative
Conformational Changes of Outer Arm-dynein Heads from Tetrahymena Cilia by Tubulin Interaction.
Author
氏名 増山 悦子
ヨミ マスヤマ エツコ
別名 Masuyama Etsuko
Abstract

Dynein couples the energy of ATP hydrolysis to drive the sliding of adjacent microtubule doublets in the axonemes of eukaryotic cilia and flagella. Due to large size of dyneins and their complex composition, it has been difficult to define the interaction of dynein and tubulin dimer. We have prepared two fragments containing the motor domain of β- and γ-heavy chins of outer arm dynein from Tetrahymena cilia. In the present study, conformational changes of the fragments were examined by using acrylodan, a cysteine-specific fluorescence probe. ATPase activities of acrylodan-modified fragments were stimulated to 2-3 times in addition to bovine brain tubulin dimer. Binding of tubulin dimer to acrylodan-modified fragments induced a large blue shift in fluorescence emission couples with ATP hydrolysis. The results of the present study indicate that dyneins cause conformational changes because acrylodan demonstrated a blue-shifted emission spectrum on exposure to a hydrophobic environment.

Journal Title
県立広島大学人間文化学部紀要
Volume
11
Spage
55
Epage
64
Published Date
20160301
Publisher
県立広島大学
ISSN
2186-5590
NCID
AA12116573
Language
jpn
NIIType
Departmental Bulletin Paper
Text Version
出版社版
Sort Key
003
Set
pu-hiroshima