A cooperative learning task was conducted among University freshmen students. Twelve female students participated in cooperative learning classes and 53 in ordinary classes. The students were asked to fill in a questionnaire before and after learning to assess their sense of self-affirmation. The results demonstrated that the students in the cooperative learning group showed higher sense of fulfillment scores than the other students after learning. In addition, the other students' sense of fulfillment scores were shown to decline throughout learning. The necessity for further investigation into the possibility for cooperative learning in higher education is suggested.